The Antibody Stains CD44+ T Cells Very Well

October 07, 2019

Pathology
Stanford
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Brilliant Violet 711™ anti-mouse/human CD44 Antibody

Catalog Number:

103057

Clone Number:

IM7

We wanted to study CD44+ T cells in the healthy vs diseased state, so we used this antibody to identify the population of interest.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Single-cell suspension of mouse spleen cells

Primary Incubation

1:200

Blocking Agent

FcBlock

Secondary Incubation

None

Tertiary Incubation

None

Detection

LSRII flow cytometer

Results Summary

The antibody identified the CD44+ T cell population very clearly.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

The antibody identifies CD44+ cells very well

The Bad

None to mention

The Bottom Line

The antibody is great for studying T cells responses in mice.

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