Good Anti-Mouse CD183 (CXCR3)

September 19, 2019

B cell memory
Deutsches Rheuma-Forschungszentrum, a Leibniz Institute
Postdoctoral Researcher

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Company:

BioLegend

Product Name:

APC anti-mouse CD183 (CXCR3) Antibody

Catalog Number:

126512

Clone Number:

CXCR3-173

Antibodies are produced by plasma cells (PC) that are terminally differentiated B cells and play crucial roles in host protection against infections but are considered to mediate autoimmunity in patients with rheumatic diseases, such as systemic lupus (SLE) or rheumatoid arthritis (RA). Our research aims at understanding the biology of antibody-secreting cells (ASC) and B cells in health and disease. Here, we aimed at staining CXCR3+ plasma cells.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse splenocytes

Primary Incubation

Surface staining with CD183 APC (1/200), 20 min at 4C

Blocking Agent

Fc block (2.4G2 clone)

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Flow cytometer

Results Summary

We specifically stained CD183 (CXCR3) plasma cells (CD138hi).

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

It works well

The Bad

N/A

The Bottom Line

Is specific and works

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