Use of SCX Spin Column to Fractionate Proteins

Havard Medical School
Dpt of Cell Bio
Postdoctoral Researcher

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Quality of Results

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Company:

Thermo Fisher Scientific

Product Name:

Pierce™ Strong Cation Exchange Spin Column

Catalog Number:

90008

These spin column were used fractionate proteins based on their charge differences prior to digestion and mass spectrometry analysis.

Experimental Design and Results Summary

Application

Protein fractionation prior to sample preparation

Starting Material

Isolated protein complex

Tips

None

Results Summary

This was used to fractionate immune-complex (protein) based on charge differences, prior to downstream application.

DOI or PMID #

N/A

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Summary

The Good

Fast and convenient

The Bad

None

The Bottom Line

These strong cation exchange spin column worked great to pre-fractionate of protein complex.

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