Trypsin Protease for Digesting Large Amount of Protein Sample for Mass Spectrometry

Havard Medical School
Dpt of Cell Bio
Postdoctoral Researcher

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Company:

Thermo Fisher Scientific

Product Name:

Pierce™ Trypsin Protease, MS Grade

Catalog Number:

90058

This products is very well suited for digesting large amount of protein for application like large scale Kgg or phospho downstream purification.

Experimental Design and Results Summary

Application

Mass Spectrometry Analysis

Starting Material

Whole cell extracts

Tips

Use in combination with LysC protease

Results Summary

Mixed Protein/Trypsin at 1:100 ratio (ug).After 6h incubation at 37ºC, all proteins were fully digested.

DOI or PMID #

10.1074/jbc.ra119.009654

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Summary

The Good

Ease of use

The Bad

Expensive

The Bottom Line

Great products to digest large amount of proteins.

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