Good Alternative to Stained Mouse CD138

September 20, 2019

B cell memory
Deutsches Rheuma-Forschungszentrum, a Leibniz Institute
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

APC anti-mouse CD138 (Syndecan-1) Antibody

Catalog Number:

142506

Clone Number:

281-2

Antibodies are produced by plasma cells (PC) that are terminally differentiated B cells and play crucial roles in host protection against infections but are considered to mediate autoimmunity in patients with rheumatic diseases, such as systemic lupus (SLE) or rheumatoid arthritis (RA). Our research aims at understanding the biology of antibody-secreting cells (ASC) and B cells in health and disease. Here, we aimed at staining plasma cells (CD138hi).

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

C57BL/6 spleen and bone marrow

Primary Incubation

Surface staining with CD138 APC (1/200), 20 min at 4C

Blocking Agent

Fc block (2.4G2 clone)

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Flow cytometer

Results Summary

We could stain plasma cells - CD138hi in spleen and BM.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

It works

The Bad

Not as good (bright) as CD138BV421

The Bottom Line

It works

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