BioLegend
PerCP/Cy5.5 anti-mouse/human CD45R/B220 Antibody
103236
RA3-6B2
Info:Supplier Page View Product SpecsView Product
Browse Similar Products: B220 Flow Cytometry Antibodies
Antibodies are produced by plasma cells (PC) that are terminally differentiated B cells and play crucial roles in host protection against infections but are considered to mediate autoimmunity in patients with rheumatic diseases, such as systemic lupus (SLE) or rheumatoid arthritis (RA). Our research aims at understanding the biology of antibody-secreting cells (ASC) and B cells in health and disease. Here, we aimed at staining B cells (CD19+B220+) in mouse spleen.
Flow Cytometry
WT (B6) spleen
Surface staining with anti-B220 Percp-Cy55, 20 min at 4C
Fc block (2.4G2 clone)
N/A
Flow cytometer
We could detect B cells (CD19+B220+). Only B1 cells do not express B220. Also, we can clearly detect B220+ and B220- plasma cells.
It works very well