Santa Cruz Biotechnology
Parkin Antibody (PRK8)
sc-32282
PRK8
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This antibody is used routinely to immunoblot extracts from HeLa, HEK293, and neurons.
Western Blot
Induced-neuron cells extracts
1:2000 overnight incubation at 4ºC, in 5% BSA diluted TBS+Tween (0.25%)
10% non-fat milk in TBS+Tween (0.25%) for 10 min
1:20000, 1 h incubation at room temperature in 5% non-fat milk diluted TBS+Tween (0.25%)
Washed three times with TBS+Tween (0.25%) for 3 min each.
HRP-based chemiluminescent immunoblotting and x-ray film
This antibody was used to immunoblot for Parkin in neuron extracts from wild-type and ko background
10.1016/j.molcel.2018.03.012
N/A
Works very well and is specific
None
This is my favorite Parkin antibody and we use it across several cell lines.