Abcam
Anti-Mitofusin 2 antibody
ab124773
NIAR164
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Browse Similar Products: Mitofusin 2 Western Blot Antibodies
This antibody was used to probe for the level of MFN2 but also to detect ubiquitylated forms of MFN2. This antibody has been very useful for this application (immunoblotting).
Western Blot
HeLa cells extracts enriched for ubiquitylated proteins
1:1000 overnight incubation at 4ºC, in 5% BSA diluted TBS+Tween (0.25%)
10% non-fat milk in TBS+Tween (0.25%) for 10 min
1:20000, 1 h incubation at room temperature in 5% non-fat milk diluted TBS+Tween (0.25%)
Washed three times with TBS+Tween (0.25%) for 3 min each.
HRP-based chemiluminescent immunoblotting and x-ray film
This antibody worked very well and was able to specifically detect the signal of interest and the ubiquitylated form of the protein.
10.1016/j.molcel.2014.09.007
N/A
Great sensitivity and specificity
None
I highly recommend people to use this antibody for this application.