Santa Cruz Biotechnology Inc
CD36 Antibody
sc-7309
SMf
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The time course effect of a growth factor treatment on CD36 protein levels was measured in human endothelial cells. In brief, the growth synchronized cells were exposed to a growth factor for the indicated period, and the whole-cell extract was prepared. After protein quantification, an equal amount of proteins was resolved in SDS-PAGE and western transferred. The transferred proteins in the nitrocellulose membrane were immunoprobed with CD36 antibody obtained from Santacruz Biotechnology Inc. The antibody selection was based on flyer information.
Western Blot
The human whole cell lysate
Dilution (1:250) in 5% skimmed milk prepared in 1x TBST, overnight incubation at 4 degree Celsius
5% skimmed milk made in 1x TBST
Goat anti-mouse IgG HRP Ab, dilution (1:8000) in 5% skimmed milk made in 1x TBST, 1hr at RT
None
Enhanced chemiluminescent detection in X-Ray films
A highly specific and bright protein band of its desired molecular weight was detected. However, no effect of the growth factor on CD36 protein levels was observed.
N/A
Detected only specific band of CD36
Excellent antibody for CD36 detection