Abcam
Anti-Notch2 antibody
ab8927
Polyclonal
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Browse Similar Products: Notch2 Western Blot Antibodies
Aim of the study was to test the effect of an on-target siRNA on Notch2 protein levels in human cells. The transfected cells were transfected with on-target siRNA, and after incubation for 36 hrs, the cultured cells were synchronized and treated with a growth factor. The cells were lysed, protein extracts were made and resolved in 10% SDS-PAGE. The resolved proteins were immunoblotted with Notch-2 antibody to detect its levels. Selection of antibody was based on information provided in antibody flyer.
Western Blot
Human smooth muscle protein extract
Dilution (1:1000) in 5% skimmed milk prepared in 1x TBST, overnight incubation at 4 degree Celsius
5% skimmed milk made in 1x TBST
Goat anti-rabbit IgG HRP Ab, dilution (1:4000) in 5% skimmed milk made in 1x TBST, 1hr at RT
None
Enhanced chemiluminescent detection in X-Ray films
Abcam NOTCH-2 antibody worked very well and detected only one highly specific, a bright band of Notch2.
N/A
Gives highly specific single bright band
Excellent Notch-2 antibody