Antibody for KLHL7

August 26, 2019

Pharmacology and Oncology
Johns Hopkins School of Medicine
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Atlas Antibody

Product Name:

Anti-KLHL7 antibody produced in rabbit

Catalog Number:

HPA029491-100UL

Clone Number:

Polyclonal

I tested this antibody for western blot using HEK293 cell lysate. There are many nonspecific bands.

Experimental Design and Results Summary

Applications

Western Blot

Sample

HEK293T

Primary Incubation

1;500, 4 degree, overnight

Blocking Agent

5% milk

Secondary Incubation

1:2000 room temp 1 hour

Tertiary Incubation

None

Detection

ECL

Results Summary

There are many nonspecific bands. The signal is not very strong.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Bands at right molecular weight was detected

The Bad

Not specific

The Bottom Line

I don't recommend this antibody as it is not specific enough and the results are difficult to analyze

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