Abcam
Anti-S6K1 (phospho T229) antibody
ab59208
Polyclonal
Info:Supplier Page View Product SpecsView Product
Browse Similar Products: S6K1 Flow Cytometry Antibodies
Aim of the study was to test the effect of treatment of a growth factor to cells on phospho S6K1 (T229) levels. The cells were treated with the growth factor for the indicated period. The S6K1 (phospho T229) levels were measured using anti-S6K1 (phospho T229)antibody procured from Abcam. The selection of antibody from Abcam was based on the western blot picture of antibody specificity, which was shown in the antibody flyer.
Western Blot
Human cell line whole-cell protein lysate
Dilution (1:1000) in 5% skimmed milk prepared in 1x TBST, overnight incubation at 4 degree Celsius
5% skimmed milk made in 1x TBST
Goat anti-mouse IgG HRP Ab, dilution (1:5000) in 5% skimmed milk prepared in 1x TBST, 1hr at RT
None
Enhanced chemiluminescent detection in X-Ray films
In the present study, an antibody from Abcam was used for immunodetection. This antibody has shown excellent specificity towards S6K1 (phospho T229) in human cells. Moreover, growth factor treatment to human cell line affected the S6K1 (phospho T229) study state levels.
N/A
Detected highly specific band of S6K1 (phospho T229)
Excellent antibody