BioLegend
APC anti-mouse TCR β chain Antibody
109212
H57-597
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MC38 tumors were excised from B6 mice and digested with Collagenase IV (1 mg/ml) and DNaseI (200 ug/ml). Cells were then stained with L/D Blue for gating out dead cells. Subsequently, cells were stained with a range of different antibodies to identify a range of immune cell types. TCRb-apc from Biolegend was one of the antibodies used at a 1:200 dilution. Antibodies were incubated with the tumor cell suspension for 30 minutes at 4 deg C.
Flow Cytometry
Mouse Tumor
1:200
Fc block
N/A
Flow Cytometry (BD Fortessa)
TCRb+ cells could be clearly gated after tumor cell suspension was stained with antibodies to identify immune cells. First, cells were gated on CD45 and then output for TCRb- apc (1:200).
Stains quite bright
Good antibody for staining mouse TCRb+ cells.