BioLegend
Pacific Blue™ anti-mouse/human CD11b Antibody
101224
M1/70
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MC38 tumors were excised from B6 mice and digested with Collagenase IV (1 mg/ml) and DNaseI (200 ug/ml). Cells were then stained with L/D Blue for gating out dead cells. Subsequently, cells were stained with a range of different antibodies to identify a range of immune cell types. CD11b-pacfic blue from BioLegend was one of the antibodies used at a 1:200 dilution. Antibodies were incubated with the tumor cell suspension for 30 minutes at 4 deg C.
Flow Cytometry
Mouse Tumor
1:200
Fc block
N/A
Flow Cytometry (BD Fortessa)
CD11b+ cells could be clearly gated after tumor cell suspension was stained with antibodies to identify immune cells. First, cells were gated on CD45 and then output for CD11b- pacific blue (1:200).
Stains quite bright
Good antibody for staining both mouse and human CD11b+ cells.