BioLegend
Brilliant Violet 510™ anti-mouse CD45 Antibody
103138
30-F11
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MC38 tumors were excised from B6 mice and digested with Collagenase IV (1mg/ml) and DNaseI (200 ug/ml). Samples were processed further and stained with L/D blue to gate out dead cells. Subsequently, anti-CD45 antibody from Biolegend (clone: 30-F11) was used at a 1:200 dilution for 30 minutes at 4 deg C. Cells were then washed with wash buffer and analyzed by BD Fortessa.
Flow Cytometry
Mouse Tumor
1:200
Fc block
N/A
Flow Cytometry (BD Fortessa)
There was a very good separation of CD45+ cells from a mixture of tumor cell suspension.
Stains very bright
Great antibody to use for identifying immune cells in cell suspensions with mixed cell types.