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Brilliant Violet 510 anti-mouse CD3ε Antibody
100353
145-2C11
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Browse Similar Products: CD3 Flow Cytometry Antibodies
Antibodies are produced by plasma cells (PC) that are terminally differentiated B cells and play crucial roles in host protection against infections but are considered to mediate autoimmunity in patients with rheumatic diseases, such as systemic lupus (SLE) or rheumatoid arthritis (RA). Our research aims at understanding the biology of antibody-secreting cells (ASC) and B cells in health and disease. Here we use anti-CD3 to exclude T cells from our analysis.
Flow Cytometry
Peritoneal Cavity cells from a C57Bl/6
Surface staining with anti-CD19, anti-CD5 and anti-CD3 BV510 (1/100), 20 min at 4C
Fc block (2.4G2 clone)
N/A
Flow cytometer with a violet laser
As it can be seen in the figure, not all T cells (CD5hi in red) are stained and the staining is very weak. At least it seems to be specific and no CD19+ cells are stained.
Is specific
Very weak staining
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