Thermo Fisher Scientific
Lipofectamine 2000 Transfection Reagent
11668027
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1 μg of ER-localized TRICA-mCherry DNA and 1.5 μl lipofectamine 2000 were diluted separately in 100 μl Optimem. After 5 min, diluted DNA was added to diluted lipofectamine 2000 and incubated for 15 min. The complex was added to HEK293 cells (around 70-80% confluent) in 35-mm dish and incubated for 8 h.
DNA transfection into cell lines.
DNA plasmid
Don't incubate DNA-lipid complex for more than 30 min.
The transfected cells were visualized after 24 h for mCherry fluorescence, localized in the endoplasmic reticulum.
N/A
Easy and efficient transfection of HEK293 cells.
Cells are stressed when incubated with transfection mixture for more than 24 h.
Lipofectamine 2000 is efficient in transfecting DNA into HEK293 cells.