The Good
It seems to stain
The Bad
Stain is very dim, difficult to get positive control
The Bottom Line
Not sure if this is actually staining HMGB1, since none of the death controls seemed to elevate the MFI of HMGB1 in a significant way. However, it does seem that hypoxia may slightly elevate the expression of HMGB1. This antibody was a little difficult to validate.