R&D Systems
Mouse EOMES Antibody
MAB8889
1219A
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Browse Similar Products: EOMES Immunofluorescence Antibodies
We study PyMT-driven mammary gland tumors. To detect NK cells in tumors, we tested this monoclonal rabbit a-Eomes antibody in tumors, with excellent results.
Immunofluorescence
PLP fixed mouse (C57BL/6) PyMT tumor cryosections
1 in 500 for 3 hr at room temperature
1% BSA, 1% FCS, 0.3% Triton X, 2% mouse serum, 2% rat serum, 2% goat serum
Goat anti-rabbit Alexa Fluor 647 (1:500) 1hr at room temperature
N/A
Images were acquired using an inverted Zeiss LSM880 confocal microscope with an 20x 0.8NA air objective.
Clear nuclear Eomes staining: Eomes (red), CD45 (green), Hoechst (blue)
Very nice staining
None
Excellent anti-Eomes antibody for IF.