R&D Systems
Mouse Integrin alpha E/CD103 Antibody
AF1990
N/A
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Browse Similar Products: Integrin Alpha E Immunofluorescence Antibodies
We study tumorigenesis in the PyMT-driven mammary gland tumor mouse model. Before visualizing tissue resident lymphocytes in tumors, we tested this polyclonal goat a-Itgae antibody in small intestine as a positive control, with very good results.
Immunofluorescence
PLP fixed mouse (C57BL/6) small intestine cryosections
1 in 100 for 3 hr at room temperature
1% BSA, 1% FCS, 0.3% Triton X, 2% mouse serum, 2% rat serum, 2% donkey serum
Donkey anti-goat Alexa Fluor 647 (1:500) for 1 hr at room temperature
Images were acquired using an inverted Zeiss LSM880 confocal microscope with an 20x 0.8NA air objective.
Very nice staining of Itgae+ cells: CD3 (green), Itgae (red), Hoechst (blue).
Reliable & reproducible staining
None
An excellent goat polyclonal antibody against mouse Itgae.