Miltenyi CD177 on Human Peripheral Blood Neutrophils

August 16, 2019

Institute for experimental Immunology and Imaging
University Hospital Essen, University of Duisburg-Essen
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Miltenyi Biotec

Product Name:

CD177-PE, human

Catalog Number:

130-101-523

Clone Number:

REA258

We want to investigate the expression of CD177 on human peripheral blood neutrophils in health and disease, as well as other markers to determine their activation status.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Purified human peripheral blood neutrophils

Primary Incubation

1:11, 4°C, dark in 100 µl of PBS for 15 min

Blocking Agent

None

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

MACSQuant VYB

Results Summary

CD177 is expressed by a large portion of CD15+, CD16+ purified, peripheral blood neutrophils. CD177 produced two distinct populations and the isotype control was negative.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

The product was well titrated and worked in the given concentration at first try.

The Bad

Nothing, as far as we can say.

The Bottom Line

The CD177 antibody by Miltenyi Biotec worked like a charm for us. It worked without large titrations and blockings on our human peripheral blood neutrophils and we will be able to go on with our experiments sooner than expected.

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