Abcam
Recombinant Anti-SOX9 antibody [EPR14335-78] (ab185966)
ab185966
EPR14335-78
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The aim of our research is to study neuroinflammation in animal models with neurodegenerative disease. The specific goal of this experiment was to quantify astrocytes. This antibody provided excellent staining for mouse optic nerve.
Immunofluorescence
Mouse optic nerve
Diluted at 1:100, overnight at 4°C
10% normal donkey serum + 0.5% triton X + 0.1M PBS
Alexa 488 conjugated anti-rabbit IgG 1:500, 1 hr at RT
N/A
Fluorescence
Clearly labeled astrocytes in mouse optic nerve tissues.
Specific nuclear labeling
Relatively high background
Good astrocytic marker in mouse central nervous system.