A Good Astrocyte Marker in the Optic Nerve - Abcam

August 15, 2019

Neuroscience
Wisconsin University
Postdoctoral Researcher

Overall

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Company:

Abcam

Product Name:

Recombinant Anti-SOX9 antibody [EPR14335-78] (ab185966)

Catalog Number:

ab185966

Clone Number:

EPR14335-78

The aim of our research is to study neuroinflammation in animal models with neurodegenerative disease. The specific goal of this experiment was to quantify astrocytes. This antibody provided excellent staining for mouse optic nerve.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Mouse optic nerve

Primary Incubation

Diluted at 1:100, overnight at 4°C

Blocking Agent

10% normal donkey serum + 0.5% triton X + 0.1M PBS

Secondary Incubation

Alexa 488 conjugated anti-rabbit IgG 1:500, 1 hr at RT

Tertiary Incubation

N/A

Detection

Fluorescence

Results Summary

Clearly labeled astrocytes in mouse optic nerve tissues.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Specific nuclear labeling

The Bad

Relatively high background

The Bottom Line

Good astrocytic marker in mouse central nervous system.

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