Great Granzyme C Antibody for Immunofluorescence (BioLegend)

August 19, 2019

Immunology
MSKCC
Research Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Purified anti-mouse Granzyme C Antibody

Catalog Number:

150802

Clone Number:

SFC1D8

We study tumorigenesis in PyMT model. We tested this monoclonal Hamster a-Granzyme C antibody in salivary gland, as a positive control, with very good results.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

PLP fixed mouse (C57BL/6) salivary gland cryosections

Primary Incubation

1 in 100 for 3 hr at room temperature

Blocking Agent

1% BSA, 1% FCS, 0.3% Triton X, 2% mouse serum, 2% rat serum, 2% goat serum

Secondary Incubation

Goat anti-Armenian Hamster Alexa Fluor 647 (1:500)

Tertiary Incubation

N/A

Detection

Images were acquired using an inverted Zeiss LSM880 confocal microscope with an 20x 0.8NA air objective.

Results Summary

Good staining of Granzyme C+ cells: CD45 (green), Granzyme C (red), Hoechst (blue).

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Very good staining of Granzyme C

The Bad

None

The Bottom Line

One of the few (if not the only) antibody for staining Granzyme C by IF.

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