PCR Master Mix for Superior Performance for a Broad Range of Amplicons (NEB)

Folium Food Science
Microbial Product Development
Group/Project Leader

Overall

Quality of Results

Ease-of-Optimization

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Company:

NEB

Product Name:

Q5® High-Fidelity 2X Master Mix

Catalog Number:

M0492S

We are using this PCR master mix for a broad range of amplicon (1kb to 15kb) PCR reaction from any kind of template. We selected this product because it's easy to use without doing any optimization; in case of bigger size amplicon may be required to do gradient PCR for single band amplification. It works well for my all kind of PCR, so far and I have been using it for 6 years.

Experimental Design and Results Summary

Application

Superior performance for a broad range of amplicons

Starting Material

DNA

Tips

Template should be less 1pg to 10ng

Results Summary

Amplification up to 10kb amplify very well and got a single band

DOI or PMID #

N/A

Image Gallery

Summary

The Good

Specific band

The Bad

Expensive

The Bottom Line

It works well for all kinds of PCR.

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