Thermo Fisher Scientific
Fixable viability dye eFlour 506
65-0866-14
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Studying the role of immune cells in antibody response during their encounter to an antigen. This fixable viability dye was used to irreversibly stain the dead cells present in the C57B/L6 mice splenocytes prior to cell sorting, fixation and analysis. Staining splenocytes with this dye helps in correct analysis by excluding dead cells population from the total splenocytes. Cells stained with this dye get excited by violet laser (405 nm) and detected by amcyan filter.
Flow cytometry
Splenocytes
Can be used for any species cells at 1:1000 dilution
Clear cloud of cells stained with this dye observed in the amycan filter, indicating dead cell population.
N/A
Easy to use, brightly stain dye
Requires compensation when pacific blue dye present in the cocktail
Recommends this dye for all the flow cytometry analysis. Use at 1:1000 dilution for best results.