Good BioLegend Antibody for IRF4 in Humans

CCTI
Columbia University
Lab Manager / Research Associate

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

PerCP/Cy5.5 anti-IRF4 Antibody

Catalog Number:

646416

Clone Number:

IRF4.3E4

Our lab studies the T cells throughout different human tissue at different ages and other conditions. We stimulate these T cells under different models and needed a good antibody to look at IRF4 expression in each cell population. We chose this antibody because it is bright and stable.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human lung cells

Primary Incubation

Cells were fixed in Tonbo fix/perm buffer and then stained at 1:50 in perm buffer for 30 minutes at room temperature.

Blocking Agent

FACS

Secondary Incubation

None

Tertiary Incubation

None

Detection

LSR II

Results Summary

This antibody works well to show us positive and negative expression of IRF4.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Bright and stable

The Bad

None

The Bottom Line

This is a good antibody for IRF4 staining in human T cells.

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