Easy Visualization for TIRF and Widefield Microscopy - Olympus

August 01, 2019

Medical University of Graz
Biophysics
Postdoctoral Researcher

Overall

Performance

Ease-of-Optimization

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Company:

Olympus

Product Name:

cell^TIRF Illuminator

Catalog Number:

cell^TIRF 1.3

Based on the optical parameters (60x/1.49 oil objective, refractive indices 1.515 (glass), 1.36 (sample)), critical angle and penetration depth can be calculated and set for all 4 laser units (445, 514, 561 and 405 nm) connected to Olympus IX81 motorized inverted microscope.

Experimental Design and Results Summary

Application

Epifluorescence and TIRF imaging

Tips

Open the software after starting the TIRF controller to avoid problem with connection.

DOI or PMID #

N/A

Image Gallery

Summary

The Good

Quick and independent setting of TIRF angle for all 4 lasers.

The Bad

Sometimes, the software doesn't respond to switching between epifluorescence and TIRF planes.

The Bottom Line

User-friendly control of epifluorescence and TIRF planes independently for 4 laser lines.

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