Abcam
Anti-IL-32 antibody
ab37158
Polyclonal
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Kinetic binding analysis performed via biacore indicates that recombinant human IL-32 (RnD systems) does not bind Abcam Rabbit Anti CD206 PAb (ab37158) immobilized directly to chip.
Biacore binding analysis
Rabbit anti-IL-32 polyclonal Ab ab37158
Abcam pAb 37158 immobilized at 20ug/ml to 1000 RU density on CM5 chip
N/A
huIL-32 at 900m titrated to 1nM associate 4min and disscociate 10min
Surface Plasmon Resonance
Human IL-32 did not bind to directly immobilized ab37158
The IgG format can allow for capture
It did not bind huIL32
The reagent does not work in binding assay when it is directly immobilized on chip.