Anti-IL-32 Antibody from Abcam Does Not Bind Recombinant Human IL-32 Beta

July 18, 2019

Biologics
AbbVie
Research Scientist

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Company:

Abcam

Product Name:

Anti-IL-32 antibody

Catalog Number:

ab37158

Clone Number:

Polyclonal

Kinetic binding analysis performed via biacore indicates that recombinant human IL-32 (RnD systems) does not bind Abcam Rabbit Anti CD206 PAb (ab37158) immobilized directly to chip.

Experimental Design and Results Summary

Applications

Biacore binding analysis

Sample

Rabbit anti-IL-32 polyclonal Ab ab37158

Primary Incubation

Abcam pAb 37158 immobilized at 20ug/ml to 1000 RU density on CM5 chip

Blocking Agent

N/A

Secondary Incubation

huIL-32 at 900m titrated to 1nM associate 4min and disscociate 10min

Tertiary Incubation

N/A

Detection

Surface Plasmon Resonance

Results Summary

Human IL-32 did not bind to directly immobilized ab37158

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

The IgG format can allow for capture

The Bad

It did not bind huIL32

The Bottom Line

The reagent does not work in binding assay when it is directly immobilized on chip.

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