Elevation of Nitrite Levels in LPS-Treated Macrophages

Medical University of Graz
Stiftingtalstraße 24
Postdoctoral Researcher

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Company:

R&D Systems

Product Name:

Total Nitric Oxide and Nitrate / Nitrite ELISA

Catalog Number:

KGE001

Elevated plasma nitrite levels initiate detrimental effects on vascular system. We used this kit to determine nitrite levels in RAW macrophages after LPS treatment (1 microg/ml). We observed a significant increase in nitrite levels after LPS treatment that were reversed by empagliflozin treatment.

Experimental Design and Results Summary

Application

Cell culture

Starting Material

Macrophages

Tips

N/A

Results Summary

LPS treatment elevated nitrite levels in macrophages. This effect was reversed by empagliflozin treatment.

DOI or PMID #

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Summary

The Good

Worked as expected

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The Bottom Line

This kit can be used to detect nitrite levels in plasma, cells, and supernatant.

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