Good Anti-AU1 Tag Antibody from BioLegend

Genetics and Genomic Sciences
Mount Sinai
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Purified anti-AU1 Epitope Tag Antibody

Catalog Number:

901901

Clone Number:

AU1

I specialize in functional genomics and applying gene editing technologies to address immunology questions. I have recently developed a new barcoding system, which represents the largest known to date set of protein-based reporters (doi.org/10.1016/j.cell.2018.09.022). I used this product for detection of AU1-tagged recombinant protein expressed by HEK-293T cells.

Experimental Design and Results Summary

Applications

Mass cytometry

Sample

Human HEK-293T cells transduced with AU1-tagged recombinant protein

Primary Incubation

1:100 (200 ug/ml stock)

Blocking Agent

Human Fc blocking reagent (BioLegend)

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

N/A

Results Summary

Good antibody for detection of AU1-tagged recombinant proteins. Low background, good specific signal.

DOI or PMID #

doi.org/10.1016/j.cell.2018.09.022

Additional Notes

N/A

Related Categories

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Summary

The Good

Good for mass and flow cytometry

The Bad

Need to be used at high concentration (2 ug/ml or higher)

The Bottom Line

Solid antibody, highly recommended

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