Morphological Changes in Microglia Cells After LPA Treatment

July 24, 2019

Molecular Biology and Biochemistry
Medical University of Graz
Postdoctoral Researcher

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Company:

FUJIFILM

Product Name:

Anti Iba1, Rabbit (for Immuno- cytochemistry)

Catalog Number:

019-19741

The impact of LPAR5-PKD axis on microglia morphology was studied. Primary murine microglia were treated with LPA (1µM) in the absence or presence of an LPAR5 inhibitor (TCLPA5) or a PKD family inhibitor (CRT).

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Primary murine microglia

Primary Incubation

1/100, O/N at 4°C

Blocking Agent

5% BSA

Secondary Incubation

Cy3, 1/300, 30min at 4°C

Tertiary Incubation

N/A

Detection

Confocal Microscopy

Results Summary

Inhibition of LPAR5 and PKD reverses the LPA-induced morphological changes in microglia cells.

DOI or PMID #

10.1186/s12974-017-1024-1

Additional Notes

N/A

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Image Gallery

Summary

The Good

Reliable results

The Bad

None

The Bottom Line

A standard microglial marker.

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