Localization of Alpha-Tubulin in BV2 Microglia Cells

Molecular Biology and Biochemistry
Medical University of Graz
Postdoctoral Researcher

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Company:

MilliporeSigma

Product Name:

Monoclonal Anti-α-Tubulin antibody produced in mouse

Catalog Number:

T9026

Clone Number:

DM1A

In order to visualize changes in the cytoskeleton of BV2 cells, cells were treated with LPA 1µM for 24 hours and stained for α-tubulin.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

BV2 microglia cells

Primary Incubation

1/100, 40min at 4°C

Blocking Agent

5% BSA

Secondary Incubation

Cy3, 1/300, 30min at 4°C

Tertiary Incubation

N/A

Detection

Confocal Microscopy

Results Summary

Treatment with LPA altered the morphology of BV2 microglia cells.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Very clear and reproducible results

The Bad

None

The Bottom Line

Very good antibody for visualizing the cytoskeleton of microglia cells.

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