MilliporeSigma
Monoclonal Anti-β-Actin antibody produced in mouse
A5316
AC-74
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Primary murine microglia were treated with 1µM LPA for 24 hours. Cells were stained for actin and changes in their morphology were visualized using a confocal microscope.
Immunofluorescence
Primary murine microglia
1/200, 40min at 4°C
5% BSA
Cy3, 1/300, 30min at 4°C
N/A
Confocal Microscopy
LPA treatment for 24 hours altered the morphology of microglia cells. Microglia lost their ramification and the cell bodies were increased.
Very clear and reproducible results
None
A very good antibody to observe changes in the cytoskeleton.