PeproTech
Murine RANTES (CCL5) Mini ABTS ELISA Development Kit
900-M124
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For the purposes of our studies, primary murine microglia were treated with lysophospatidic acid (LPA) 1µM in the presence or absence of a PKD family inhibitor (CRT; 1µM). Differences in levels of various pro-inflammatory cytokines and chemokines were analyzed using ELISA kits.
ELISA
Primary murine microglia
It is better to apply the coating antibody overnight and not just 2h as it is suggested in the kit's manual.
Increased levels of CCL5 were observed after LPA treatment for 2 and 8 hours. Inhibition with CRT 1µM (red bars) abrogated the LPA induced expression of CCL5.
10.1186/s12974-017-1024-1
Easy to use, reproducability of the results
Since it's not a pre-coated ELISA, the experimental procedure takes a bit longer.
Very good and reliable ELISA kit.