PeproTech
Murine IL-6 Mini TMB ELISA Development Kit
900-TM50
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Primary murine microglia were treated with lysophospatidic acid (LPA) 1µM in the presence or absence of a PKD family inhibitor (CRT; 1µM). Differences in levels of various pro-inflammatory cytokines and chemokines were analyzed using ELISA kits.
ELISA
Primary murine microglia
It is better to apply the coating antibody overnight and not just 2h as it is suggested in the kit's manual.
LPA treatment induced an increase in the expression of IL-6 after 2h. After 8 and 24h it had no effect. CRT (red bars) could decrease the LPA induced IL6 expression.
N/A
Easy to use, reproducible results
Since it's not a pre-coated ELISA, the experimental procedure takes a bit longer.
A reliable product for the detection of IL-6.