Thermo Fisher Scientific
IL-13 Monoclonal Antibody (85BRD), FITC, eBioscience™
11-7136-42
85BRD
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Browse Similar Products: IL-13 Flow Cytometry Antibodies
I tried a lot of IL-13 antibodies, and even though it's low, this is the only one I could get a consistent reasonable signal above background. The assay is finnicky and only worked using PMA and Ionomycin for 24 hours with a combination of BFA and Monensin. In addition, I had to use the eBioscience FoxP3 fix/perm buffer.
Flow Cytometry
Frozen human PBMCs
surface stain 30 min room temp (for CD markers)
PBS with 1% FCS
60 min on ice
N/A
I've attached my titration and my time course for optimizing this protocol. The negative population is pretty bright but it's tight so you can still detect a positive population.
Used eBioscience fix/perm buffer
Actually works
Low signal
IL-13 is hard to detect and this is as good as I could find.