Very Good ATF-3 Antibody from Santa Cruz

June 26, 2019

Orthopedic surgery
Johns Hopkins University School of Medicine
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Santa Cruz Biotechnology

Product Name:

Mouse anti-Atf3

Catalog Number:

sc-81189

Clone Number:

44C3a

My research focuses on molecular mechanisms of axon growth and guidance during neural development and axon regeneration after injuries to the central nervous system or peripheral nerves. For this experiment, I needed to do immunofluorescent staining on retina cryostat sections.

Experimental Design and Results Summary

Applications

Immunohistochemistry

Sample

Mouse retina cryostat sections

Primary Incubation

1:100, overnight at 4°C

Blocking Agent

PBST (0.3%) containing 10% goat serum

Secondary Incubation

1:500, 1 hr at room temperature

Tertiary Incubation

N/A

Detection

CCD camera connected to a Zeiss inverted fluorescence microscope controlled by AxioVision software

Results Summary

The immunofluorescence signal of Atf3 can be observed in the retina ganglion cells and glial cells.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

The antibody is strong

The Bad

N/A

The Bottom Line

This antibody works well.

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