Stem Cell Technologies
Easy Sept Pan-ILC enrichment kit
19875
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The frequency of ILCs in murine thymus is <1% of the live CD45+ thymocytes. In the past, I have used cell sorting to isolate thymic ILCs, but it is time consuming, expensive, and results in low purity and limited recovery of viable ILCs. Stem Cell Technologies' Easy Sep Pan-ILC enrichment kit proved to be a fast negative selection method to pre-enrich all ILCs subsets from mouse thymus. The simple to follow protocol for magnetic removal of unwanted cells allowed me to quickly transition to downstream flow cytometry applications to sort the enriched ILCs into subsets.
Cell enrichment
Mouse thymus
Requires considerable knowledge of flow cyotmetry
The pan-ILC enrichment kit enriched the ILC fraction in the thymus 10-fodl, while at the same time eliminating ~90-95% of the unwanted cells from the thymus. Together, these steps saved me considerable time and money!
N/A
Fast and easy-to-follow protocol
Even after magnetic separation, there remains a large number of lineage positive cells.
Definitely worth the costs