Good CD209 Antibody from BioLegend for IF

Dermatology
University Hospital Erlangen
Core Facility Manager

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

CD209 (DC-SIGN) Antibody

Catalog Number:

330106

Clone Number:

9E9A8

I am working with a multiplex immunofluorescence technique in which you can stain one single tissue section with more than 100 antibodies. I have a portfolio of more than 2000 different antibodies and have generated over 100,000 immunofluorescence images. I supervise several projects in which we stain different tissue samples, e.g., skin (melanoma, psoriasis), tumor, spleen, etc. For this experiment, our lab was interested in the characterization of immune cells subtypes in inflamed skin diseases like psoriasis,and ulcus cruris.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Human skin

Primary Incubation

1:50; RT; 60 min

Blocking Agent

5% FBS; RT, 30 min

Secondary Incubation

None

Tertiary Incubation

None

Detection

Fluorescence signals (PE) were detected by an inverted fluorescence microscope (Leica DM IRE2)

Results Summary

This antibody gives specific and quite strong signals on frozen-tissue sections with little background. Publishable results!

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Specific signals, easy to use!

The Bad

None

The Bottom Line

Will buy and use it again. Highly recommended!

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