Excellent GLUT1 Antibody for IF but Extremely Unspecific for Western Blot

June 19, 2019

Oncology
Johns Hopkins School of Medicine
Lab Technician

Overall

Quality of Results

Ease-of-Optimization

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Company:

Abcam

Product Name:

Anti-Glucose Transporter GLUT1 antibody (Alexa Fluor® 647)

Catalog Number:

ab195020

Clone Number:

[EPR3915]

Our lab is interested in the glucose transporter after we treat our cells with glutamine antagonism. We were wondering if the level of Glut1 was different before and after treating therefore we did western blot and IF.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Murine T cell

Primary Incubation

Overnight in 4C with 1:100 dilution

Blocking Agent

10% goat serum

Secondary Incubation

Room temperature for an hr

Tertiary Incubation

N/A

Detection

IF

Results Summary

The IF images were clear in the amount of Glut1 but it was confusing for our project because there seems to be no difference between the two conditions

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Easy to use

The Bad

The cost of the antibody is high especially if used for IF dilution

The Bottom Line

It is useful for flow cytometry but for IF it might be too costly

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