Good Brilliant Violet 785™ Anti-Human CD161 Antibody from BioLegend

Innate Immunity
Deutsches Rheuma-Forschungszentrum
PhD Candidate

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Brilliant Violet 785™ anti-human CD161 Antibody

Catalog Number:

339930

Clone Number:

HP-3G10

CD161 is a human C-type lectin and can be expressed on NK cells, NKT cells, subsets of peripheral T cells and CD3+ thymocytes, and can also be used to identify innate lymphoid cells (ILCs) across compartments.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

PBMCs

Primary Incubation

15 min, 37°c

Blocking Agent

0.5% BSA in PBS

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

FACSymphony, software FACSDiva

Results Summary

After identifying the group of interest, and negatively gating out groups of non-interest, CD161 had great resolution, particularly for NK and ILCs. Used in bulk prior to gating, resolution is low, as it is very variable within T cell and other populations. BV785 is bright, but problematic with AF700 and APCCy7.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Bright, high resolution after gating out

The Bad

Problematic with other fluorochromes, low resolution in bulk.

The Bottom Line

Would recommend, but requires optimization.

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