Good Brilliant Violet 711™ Anti-Human Ki-67 Antibody from BioLegend

Innate Immunity
Deutsches Rheuma-Forschungszentrum
PhD Candidate

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Brilliant Violet 711™ anti-human Ki-67 Antibody

Catalog Number:

350516

Clone Number:

Ki-67

Ki-67 is a nuclear protein required for cell proliferation, and we used it as a marker of proliferating lymphocytes during inflammatory disease.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

PBMCs

Primary Incubation

15 min, 37°c

Blocking Agent

0.5% BSA in PBS

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

FACSymphony, software FACSDiva

Results Summary

Proliferating cells were stained by Ki67, which could reach a high mean fluorescence intensity, however, resolution was low between positive and negative populations.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Bright, sensitive

The Bad

Low resolution between populations

The Bottom Line

It works well in BV711, but might work better in a brighter fluorochrome.

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