Decent ELISA for Detection of Human IL2 from Thermo

Georgetown University
LCB
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Thermo Fisher Scientific

Product Name:

Human IL2 Elisa

Catalog Number:

EH2IL2

This kit did a decent job for me to detect IL2 from cultures of human PBMCs. The range is pretty good and sensitivity is also great. Did not require much standardization or trials. The manufacturer's protocol was well defined and worked in most cases.

Experimental Design and Results Summary

Application

Enzyme-Linked Immunosorbent Assay

Starting Material

Human PBMCs

Tips

None

Results Summary

Good for detecting low and moderate amounts of IL2 in serum or culture supernatants from human PBMCs. Mostly used manufacturer's guideline for experiments.

DOI or PMID #

N/A

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Summary

The Good

Sensitive and reproducible

The Bad

None

The Bottom Line

Recommended for detection of human IL2 in low-abundance samples.

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