This BioLegend Antibody Stains IgD+ B Cells Well

Pathology
Stanford University
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

FITC anti-mouse IgD Antibody

Catalog Number:

405704

Clone Number:

11-26c.2a

We wanted to study IgD+ B cells in the diseased vs healthy state, so we used this antibody to stain for IgD+ B cells.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Single-cell suspension of peritoneal cells

Primary Incubation

1:200

Blocking Agent

FcBlock

Secondary Incubation

None

Tertiary Incubation

None

Detection

LSRII flow cytometer

Results Summary

The antibody stains IgD+ B cells very clearly.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

The antibody identifies IgD+ cells very well.

The Bad

None to mention

The Bottom Line

The antibody is great for looking at IgD expression on B cells.

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