This BioLegend Antibody Stains CD80+ Cells Well

May 20, 2019

Pathology
Stanford University
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Brilliant Violet 421™ anti-mouse CD80 Antibody

Catalog Number:

104726

Clone Number:

16-10A1

We wanted to look at changes in CD80 expression in the diseased vs healthy state, so we used this antibody to stain for CD80.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Single-cell suspension of peritoneal cells

Primary Incubation

1:200

Blocking Agent

FcBlock

Secondary Incubation

None

Tertiary Incubation

None

Detection

LSRII flow cytometer

Results Summary

The antibody stained CD80+ cells very clearly.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

The antibody clearly identifies CD80+ cells.

The Bad

None to mention

The Bottom Line

The antibody is great for assessing CD80 expression on cells.

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