This BioLegend Antibody Identifies CD40+ Cells Very Well

May 24, 2019

Pathology
Stanford University
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

APC anti-mouse CD40 Antibody

Catalog Number:

124612

Clone Number:

3/23

We wanted to study CD40 expression in the diseased vs healthy state, so we used this antibody to assess expression levels of CD40

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Single-cell suspension of peritoneal cells

Primary Incubation

1:200

Blocking Agent

FcBlock

Secondary Incubation

None

Tertiary Incubation

None

Detection

LSRII flow cytometer

Results Summary

The antibody stains CD40+ cells very clearly.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

The antibody clearly stains CD40+ cells.

The Bad

None to mention

The Bottom Line

The antibody is great to looking at expression levels of CD40.

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