This BioLegend Antibody Stains CD19+ Cells Well

May 17, 2019

Pathology
Stanford University
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
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Company:

BioLegend

Product Name:

APC/Cyanine7 anti-mouse CD19 Antibody

Catalog Number:

115530

Clone Number:

6D5

We wanted to study B cells in the diseased vs healthy state, so we used this antibody to identify CD19+ cells

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Single-cell suspension of mouse mesenteric lymph node cells

Primary Incubation

1:200

Blocking Agent

FcBlock

Secondary Incubation

None

Tertiary Incubation

None

Detection

LSRII flow cytometer

Results Summary

The antibody stained B cells very clearly.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

The antibody identifies B cells very well.

The Bad

None to mention

The Bottom Line

The antibody is great for identifying B cells in tissues.

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