Abcam
Anti-NDUFA9 antibody [20C11B11B11]
ab14713
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Our lab studies the role of mTOR signaling in CD8 T cell differentiation and function. mTOR signaling controls the metabolic programming of T cells. Programming of T cells also involves dynamic changes in mitochondria mass and morphology. Since effector and memory T cells have differences in these properties, we wanted to assess mitochondrial components in the electron transport chain. We used this antibody to detect NDUFA9 of Complex I.
Western Blot
Murine CD8+ T cells
1:2000 in 4%BSA/TBST 4 deg overnight with shaking
5% dry milk/TBST 1 hr room temperature with shaking
1:5000 anti-Rabbit HRP (CST) in %5 milk/TBST 1 hr room temp with shaking
None
UVP Imaging System
We are able to detect NDUFA9 of Complex I in T cell lysates. The band is at the correct molecular weight size.
N/A
Make sure gel is not run too long since this is a small protein.
Good antibody without any non-specific bands. Great to reuse diluted antibody countless times.
Great antibody to detect NDUFA9 expression in cells as a measure of mitochondrial mass.